Wang F, Su Z L, Huang J X, Wang L, Yang W Y, Jiang S C
Department of Otorhinolaryngology, PLA 304 Hospital, Beijing 100037.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2000 Aug;14(8):370-2.
To establish a culture model of human nasal respiratory epithelial cells and a method of measuring human nasal ciliary motility.
The human nasal respiratory epithelial cells were detached with collagenase and cultured in serum free medium supplemented with hormones and growth factors, the ciliary beat frequency was measured by videomicroscopy.
After inoculation, cells cultured with this method adhered in 24 hours, confluented in 6-8 days and lived for 16 days. During that time ciliary beating was active, both acidic and neutral mucoitin granules were rich in goblet cells and all chromosome of 23 pairs were normal, the ciliary beat frequency in 29 subjects' nasal mucosa was (411 +/- 24) beats/min (mean +/- s).
A culture model of human nasal respiratory epithelial cells in serum free medium supplemented with hormones and growth factors and a method of measuring human nasal ciliary motility was successfully established.
建立人鼻呼吸上皮细胞培养模型及测量人鼻纤毛运动的方法。
用胶原酶分离人鼻呼吸上皮细胞，在添加激素和生长因子的无血清培养基中培养，通过视频显微镜测量纤毛摆动频率。
接种后，用该方法培养的细胞在24小时内贴壁，6 - 8天汇合，存活16天。在此期间纤毛摆动活跃，杯状细胞内酸性和中性黏蛋白颗粒丰富，23对染色体均正常，29名受试者鼻黏膜的纤毛摆动频率为（411±24）次/分钟（均值±标准差）。
成功建立了在添加激素和生长因子的无血清培养基中人鼻呼吸上皮细胞的培养模型及测量人鼻纤毛运动的方法。
Zotero 插件