体外培养的人鼻上皮细胞中的纤毛发生及协调性纤毛摆动

Ciliogenesis and coordinated ciliary beating in human nasal epithelial cells cultured in vitro.

作者信息

Jorissen M, Van der Schueren B, Tyberghein J, Van der Berghe H, Cassiman J J

机构信息

Center for Human Genetics, University of Leuven, Belgium.

出版信息

Acta Otorhinolaryngol Belg. 1989;43(1):67-73.

PMID:2801098

Abstract

Human nasal epithelial cells, dissociated from nasal polyps, lost all their cilia when cultured as monolayers on 0.2% collagen gels in Ham's F12-DME 1/1, supplemented with NU-serum 10%, choleratoxin (10 ng/ml), retinoic acid (10(7) M) and antibiotics. These deciliated epithelial cell sheaths were then placed in a suspension culture system, and epithelial aggregates and vesicles formed. After 1 week in suspension, cilia progressively reappeared. This in vitro ciliogenesis resulted in ultrastructurally normal cilia with an intercellularly coordinated beating pattern. These epithelial aggregates and vesicles maintained this differentiated ciliary morphology and function for more than 6 months.

摘要

从鼻息肉中分离出的人鼻上皮细胞，当在补充有10% NU - 血清、霍乱毒素（10纳克/毫升）、视黄酸（10⁻⁷摩尔）和抗生素的Ham's F12 - DME 1/1培养基中的0.2%胶原凝胶上单层培养时，失去了所有纤毛。然后将这些去纤毛的上皮细胞鞘置于悬浮培养系统中，形成上皮聚集体和小泡。悬浮培养1周后，纤毛逐渐重新出现。这种体外纤毛发生导致超微结构正常的纤毛，并具有细胞间协调的摆动模式。这些上皮聚集体和小泡维持这种分化的纤毛形态和功能超过6个月。