Li X M, Yang C Z, Chen P J, Su Z L, Song Y
Department of Otolaryngology, Baoan People's Hospital, Shenzhen 518101.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2000 Sep;14(9):400-1.
Epstein-Barr virus (EBV) is associated with nasopharyngeal carcinoma (NPC). Determination of EBV-VCA-IgA, EBV-EA-IgA in serum and assay of EBV DNA (PCR) in biopsy tissue has been used for diagnosis of NPC. This paper evaluated the value of the three determinations for diagnosis of NPC.
146 patients were investigated. For each patient determination of EBV-VCA-IgA, EBV-EA-IgA in serum and EBV-DNA (PCR) in biopsy tissue were performed with double-blind studies, of 146 patients, 76 were NPC and 70 non nasopharyngeal carcinoma as control, identified with histopathological examination. The differences of the determinations between NPC and control were compared statistically with chi 2-test.
Of 76 NPC, the positive rates of EBV-DNA (PCR), EBV-VCA-IgA and EBV-EA-IgA were 90.8%, 75.0% and 26.3% respectivelly. For 70 control cases, the positive rates 2.9%, 38.6% and 2.9% respectivelly (P < 0.005).
Our results suggested that assay of EBV-DNA (PCR) has better sensitivity and specificity than EBV-VCA-IgA and EBV-EA-IgA for diagnosis of NPC.
爱泼斯坦-巴尔病毒（EBV）与鼻咽癌（NPC）相关。血清中EBV-VCA-IgA、EBV-EA-IgA的检测以及活检组织中EBV DNA（PCR）的检测已用于鼻咽癌的诊断。本文评估了这三项检测对鼻咽癌诊断的价值。
对146例患者进行研究。对每位患者进行血清中EBV-VCA-IgA、EBV-EA-IgA的检测以及活检组织中EBV-DNA（PCR）的检测，并采用双盲研究。146例患者中，76例为鼻咽癌，70例非鼻咽癌作为对照，通过组织病理学检查进行鉴定。采用卡方检验对鼻咽癌组和对照组之间各项检测结果的差异进行统计学比较。
76例鼻咽癌患者中，EBV-DNA（PCR）、EBV-VCA-IgA和EBV-EA-IgA的阳性率分别为90.8%、75.0%和26.3%。70例对照病例中，阳性率分别为2.9%、38.6%和2.9%（P<0.005）。
我们的结果表明，对于鼻咽癌的诊断，EBV-DNA（PCR）检测比EBV-VCA-IgA和EBV-EA-IgA检测具有更好的敏感性和特异性。
