[培养软骨移植修复兔生长板缺损]
[Repair of growth plate defects of rabbits with cultured cartilage transplantation].
作者信息
Wang J, Yang Z M, Xie H Q
机构信息
Department of Orthopedic Surgery, First University Hospital, West China University of Medical Sciences, Chengdu Sichuan, P. R. China 610041.
出版信息
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2001 Jan;15(1):53-6.
OBJECTIVE
To prevent early closure of growth plate and developmental deformities of limbs by allografts of cultured cartilages into growth plate defects of rabbits.
METHODS
Chondrocytes isolated from articular cartilage of 1-month rabbits formed cartilage after cultivation in centrifuge tubes. The cartilages cultured for two weeks were implanted into growth plate defects of proximal tibiae of 6-weeks rabbits. At 4th and 16th weeks, X-ray, histologic and immunohistochemical examination were performed.
RESULTS
The tibiae had no marked deformities after 4 weeks of operation. Histologic examinations showed that the defects were filled with cartilage. Immunohistochemical results of type II collagen were positive. The tibiae with allografts of cultured cartilages had no evident deformities after 16 weeks of operation. Histologic examination showed nearly closure of growth plates. On the contrary, the tibiae on control side formed severe deformities and growth plate were closed.
CONCLUSION
Allograft of cultured cartilages into growth plate defects may replace lost growth plate tissues, maintain normal growth of limbs and prevent developmental deformity.
目的
通过将培养的软骨同种异体移植到兔生长板缺损处，预防生长板过早闭合和肢体发育畸形。
方法
从1月龄兔的关节软骨分离软骨细胞，在离心管中培养后形成软骨。将培养两周的软骨植入6周龄兔胫骨近端的生长板缺损处。在第4周和第16周进行X线、组织学和免疫组织化学检查。
结果
术后4周胫骨无明显畸形。组织学检查显示缺损处充满软骨。II型胶原免疫组织化学结果为阳性。培养软骨同种异体移植的胫骨术后16周无明显畸形。组织学检查显示生长板几乎闭合。相反，对照侧胫骨形成严重畸形且生长板闭合。
结论
将培养的软骨同种异体移植到生长板缺损处可替代缺失的生长板组织，维持肢体正常生长并预防发育畸形。
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