Hedeland Ylva, Hedeland Mikael, Bondesson Ulf, Pettersson Curt
Division of Analytical Pharmaceutical Chemistry, Biomedical Centre, Uppsala University, Box 574, SE- 751 23 Uppsala, Sweden.
J Chromatogr A. 2003 Jan 17;984(2):261-71. doi: 10.1016/s0021-9673(02)01869-1.
N-Benzoxycarbonylglycyl-L-proline (L-ZGP) has been introduced as a chiral selector for enantioseparation of amines in non-aqueous capillary electrophoresis. Methanol mixed with different proportions of dichloromethane, 1,2-dichloroethane or 2-propanol containing L-ZGP and ammonium acetate was used as the background electrolyte. Enantioseparation of different types of pharmacologically active amines was performed, e.g. the local anaesthetic bupivacaine and the beta-adrenoceptor blocking agent pindolol. Addition of the solvents (dichloromethane, 1,2-dichloroethane or 2-propanol) gave an improved chiral separation partly due to a distinct decrease in the electroosmotic flow. The use of 1,2-dichloroethane in the background electrolyte gave higher precision in migration time (RSD 2.2%) compared to the systems containing dichloromethane. An enantiomeric separation of mepivacaine was performed within 72 s by use of short-end injection with an effective capillary length of 8.5 cm.
N-苄氧羰基甘氨酰-L-脯氨酸（L-ZGP）已被用作非水毛细管电泳中胺类对映体分离的手性选择剂。将含有L-ZGP和醋酸铵的甲醇与不同比例的二氯甲烷、1,2-二氯乙烷或2-丙醇混合用作背景电解质。对不同类型的药理活性胺进行了对映体分离，例如局部麻醉药布比卡因和β-肾上腺素能受体阻滞剂吲哚洛尔。添加这些溶剂（二氯甲烷、1,2-二氯乙烷或2-丙醇）部分由于电渗流明显降低而改善了手性分离。与含有二氯甲烷的体系相比，在背景电解质中使用1,2-二氯乙烷在迁移时间上具有更高的精密度（相对标准偏差为2.2%）。通过使用有效毛细管长度为8.5 cm的短端进样，在72 s内实现了甲哌卡因的对映体分离。
