锌离子对猴脑线粒体中A型单胺氧化酶的影响。
Effects of zinc ion on type A monoamine oxidase in monkey brain mitochondria.
作者信息
Egashira Toru, Takayama Fusako, Sakai Kumiko
机构信息
Department of Pharmacology, Oita Medical University, 1-1 Idaigaoka, Hasama-machi, Oita 879-5593, Japan.
出版信息
Biochem Pharmacol. 2003 Feb 15;65(4):625-7. doi: 10.1016/s0006-2952(02)01608-8.
The effects of ZnSO(4) on types A and B monoamine oxidase (MAO) isozymes in monkey brain mitochondria were investigated, in vitro. Type A MAO activity in monkey brain decreased to about 50% with 1 microM ZnSO(4) using serotonin as a substrate, and this inhibition was proportional to the concentration of ZnSO(4). ZnSO(4) had no effect, however, on type B MAO activity in monkey brain using beta-phenylethylamine as a substrate. The inhibition by ZnSO(4) of type A MAO activity was competitive and reversible. ZnSO(4) did not inhibit either type A or type B MAO activity in rat brain mitochondria. Almost similar results were also obtained when ZnCl(2) was used, in vitro. These results indicate that the inhibiting action of zinc ion differs depending on animal species and organ. Type A MAO in monkey brain mitochondria was highly sensitive to zinc ion, while type B activity was less sensitive.
在体外研究了硫酸锌（ZnSO₄）对猴脑线粒体中A、B型单胺氧化酶（MAO）同工酶的影响。以血清素为底物时，用1微摩尔硫酸锌处理后，猴脑A型MAO活性降至约50%，且这种抑制作用与硫酸锌浓度成正比。然而，以β-苯乙胺为底物时，硫酸锌对猴脑B型MAO活性无影响。硫酸锌对A型MAO活性的抑制是竞争性且可逆的。硫酸锌对大鼠脑线粒体中的A型或B型MAO活性均无抑制作用。在体外使用氯化锌（ZnCl₂）时也得到了几乎相似的结果。这些结果表明，锌离子的抑制作用因动物物种和器官而异。猴脑线粒体中的A型MAO对锌离子敏感，而B型活性则不太敏感。
Zotero 插件