Su Song-Nan, Peng Ho-Jen, Yang Sue-Yee, Tsai Lai-Chen, Chow Lu-Ping, Huang Shih-Wen
Department of Medical Research and Education, Veterans General Hospital-Taipei, Taipei, Taiwan, ROC.
J Biomed Sci. 2003 Jan-Feb;10(1):111-9. doi: 10.1007/BF02256003.
A novel immunoreactive isoallergen of a major Bermuda grass pollen allergen, Cyn d 1, was purified by the use of a combination of various chromatographic techniques, including high-performance liquid chromatography. This new isoallergen has a pI value of 9.1 and shows significant N-terminal sequence homology with other isoforms. Carbohydrate composition analysis revealed a 10.4% carbohydrate content consisting of 7 different sugar moieties, including arabinose, fucose, galactose, glucose, mannose, xylose and N-acetylglucosamine, as well as a trace amount of rhamnose. Upon periodate oxidation, the binding activities of the Cyn d 1 isoform to murine monoclonal antibodies and human serum IgE and IgG were reduced, suggesting the importance of the carbohydrate moiety in the immune response. The availability of the purified Cyn d 1 basic isoform will allow for further structural and immunological characterization, and ultimately for the design of an appropriate therapy.
利用包括高效液相色谱在内的多种色谱技术组合，纯化出了一种新型的百慕大草主要花粉过敏原Cyn d 1的免疫反应性同种变应原。这种新的同种变应原的pI值为9.1，与其他同种型显示出显著的N端序列同源性。碳水化合物组成分析显示，其碳水化合物含量为10.4%，由7种不同的糖部分组成，包括阿拉伯糖、岩藻糖、半乳糖、葡萄糖、甘露糖、木糖和N-乙酰葡糖胺，以及微量的鼠李糖。经高碘酸盐氧化后，Cyn d 1同种型与鼠单克隆抗体以及人血清IgE和IgG的结合活性降低，表明碳水化合物部分在免疫反应中的重要性。纯化的Cyn d 1碱性同种型的可得性将有助于进一步进行结构和免疫学表征，并最终用于设计合适的治疗方法。
