Liu Xiao-mei, Zhang Hong-quan
Medical and Pharmacological Institute of Yangzhou University, Yangzhou 225001, China.
Yao Xue Xue Bao. 2002 Aug;37(8):616-20.
To evaluate the effect of polysaccharide from Spirulina platensis (PSP) on hematopoietic cell proliferation, apoptosis and Bcl-2 expression in mice bearing tumor treated with chemotherapy.
The model of chemotherapy for transplant solid tumor in mice was established. The hematopoietic cell proliferation, apoptosis, Bcl2 expression and related cytokines were assayed by the technique of culture of hematopoietic progenitor cell, fluoromicroscope and light microscope, immunohistochemical method, and double antibody sandwich ELISA.
PSP significantly ameliorated CFU-GM proliferation inhibition and hematopietic cells apoptosis induced by CTX. Moreover, PSP evidently increased the content of IL-1, IL-3, GM-CSF and TNF-alpha in serum and Bcl-2 expression of hematopoietic cells.
PSP indirectly upregulated Bcl-2 expression of hematopoietic cells by promoting endogenous cytokines secretion which may be one of the mechanisms, by which PSP enhanced hematopoietic cell proliferation and inhibited its apoptosis in mice bearing tumor treated with chemotherapy.
评估钝顶螺旋藻多糖（PSP）对化疗荷瘤小鼠造血细胞增殖、凋亡及Bcl-2表达的影响。
建立小鼠移植性实体瘤化疗模型。采用造血祖细胞培养技术、荧光显微镜和光学显微镜、免疫组化方法及双抗体夹心ELISA法检测造血细胞增殖、凋亡、Bcl-2表达及相关细胞因子。
PSP显著改善环磷酰胺（CTX）诱导的CFU-GM增殖抑制和造血细胞凋亡。此外，PSP明显增加血清中IL-1、IL-3、GM-CSF和TNF-α的含量以及造血细胞的Bcl-2表达。
PSP通过促进内源性细胞因子分泌间接上调造血细胞Bcl-2表达，这可能是PSP增强化疗荷瘤小鼠造血细胞增殖并抑制其凋亡的机制之一。
