Yang T, Ge L, Wan X
Laboratory of Neurobiology, Institute of Basic Medical Sciences of Hebei Province, Hebei Medical University, Shijiazhuang 050017.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1999 Jun;21(3):214-20.
In order to investigate how widespread the activated regions induced by an olfactory stimulus throughout the central nervous system (CNS), c-fos expressions in CNS of the rat observed, which is known as a combined morphologically and functionally probe.
Rats were stimulated by iso-amyl acetate for about 10 times, then one hour later sacrificed with perfusion of fixatives. After ABC immunocytochemi cal staining with anti-c-fos polyclonal antiserum, the c-Fos protein labelling areas on sections of the brain were observed.
The results showed that the labelling areas were estremely widespread throughout the brain and cervical segments of the spinal cord in the rat, expecially much more in the areas above the level of the pons. Nearly all neuronal structures in the limbic system were labeled in addition to structures of the olfactory pathways, suggesting there were neuronal activities in multiple neural circuits, which may be associated with emotional, somatic and visceral responses to the olfactory stimulus. Next, many brain areas which were associated with mechanisms of attention and central state regulation were widely labeled. Unexpected heavy labellings in deep layers of the superior colliculus, inferior colliculus and pontine nuclei were presumably relevant to integrating activity between sensations and motions.
The present study indicated that a specific olfactory stimulus did induce widespread activities of so-called pan-brain networks within the central nervous system.
为了研究嗅觉刺激在中枢神经系统（CNS）中所诱导的激活区域有多广泛，观察了大鼠中枢神经系统中c-fos的表达情况，c-fos是一种形态学和功能相结合的检测指标。
用乙酸异戊酯刺激大鼠约10次，1小时后灌注固定液处死。用抗c-fos多克隆抗血清进行ABC免疫细胞化学染色后，观察脑切片上的c-Fos蛋白标记区域。
结果表明，大鼠脑和脊髓颈段的标记区域极为广泛，尤其是脑桥水平以上的区域。除嗅觉通路结构外，边缘系统中几乎所有神经元结构均被标记，提示多个神经回路存在神经元活动，这可能与对嗅觉刺激的情绪、躯体和内脏反应有关。其次，许多与注意力机制和中枢状态调节相关的脑区被广泛标记。上丘、下丘和脑桥核深层意外出现的大量标记可能与感觉和运动之间的整合活动有关。
本研究表明，特定的嗅觉刺激确实能在中枢神经系统内诱导所谓的全脑网络的广泛活动。
