Harris Ashley C, Wheeler John F
Forensics Laboratory, Spartanburg County Sheriff's Office, Sparmburg, SC, USA.
J Forensic Sci. 2003 Jan;48(1):41-6.
Solid-phase microextraction (SPME) is well documented with respect to its convenience and applicability to sampling volatiles. Nonetheless, fire debris analysts have yet to widely adopt SPME as a viable extraction technique, although several fire debris studies have demonstrated the utility of SPME coupled with gas chromatography-mass spectrometry (GC-MS) to identify ignitable liquids. This work considers the expansion of SPME sampling from the customary thermal desorption mode to solvent-based analyte desorption for the analysis of ignitable residues. SPME extraction fibers are desorbed in 30 microL of nonaqueous solvent to yield a solution amenable to conventional GC-MS analysis with standard autosampler apparatus. This approach retains the advantages of convenience and sampling time associated with thermal desorption while simultaneously improving the flexibility and throughput of the method. Based on sampling results for three ignitable liquids (gasoline, kerosene, anddiesel fuel) in direct comparisons with the widely used activated charcoal strip (ACS) method this methodology appears to be a viable alternative to the routinely used ACS method.
固相微萃取（SPME）在其便利性以及对挥发性物质采样的适用性方面已有充分记载。尽管如此，火灾残骸分析人员尚未广泛采用SPME作为一种可行的萃取技术，尽管多项火灾残骸研究已证明SPME与气相色谱-质谱联用（GC-MS）在识别可燃液体方面的实用性。这项工作考虑将SPME采样从传统的热解吸模式扩展到基于溶剂的分析物解吸，用于分析可燃残留物。SPME萃取纤维在30微升非水溶剂中解吸，以产生适合使用标准自动进样器进行常规GC-MS分析的溶液。这种方法保留了与热解吸相关的便利性和采样时间优势，同时提高了该方法的灵活性和通量。基于对三种可燃液体（汽油、煤油和柴油）的采样结果，并与广泛使用的活性炭条（ACS）方法进行直接比较，该方法似乎是常规使用的ACS方法的一种可行替代方案。
