Harakava Ricardo, Gabriel Dean W
Department of Plant Pathology, University of Florida, Gainesville, Florida 32611, USA.
Appl Environ Microbiol. 2003 Feb;69(2):1315-9. doi: 10.1128/AEM.69.2.1315-1319.2003.
Suppression subtractive hybridization was used to rapidly identify 18 gene differences between a citrus variegated chlorosis (CVC) strain and a Pierce's disease of grape (PD) strain of Xylella fastidiosa. The results were validated as being highly representative of actual differences by comparison of the completely sequenced genome of a CVC strain with that of a PD strain.
抑制性消减杂交技术被用于快速鉴定桑氏假单胞菌柑橘杂色黄化病(CVC)菌株与葡萄皮尔氏病(PD)菌株之间的18个基因差异。通过将CVC菌株的全基因组序列与PD菌株的全基因组序列进行比较,验证了这些结果高度代表实际差异。