[Random amplified polymorphic DNA analysis of Cryptosporidium species and strains].
作者信息
Tian Z C, Zhang X C, Wang J M, Yin J G, Li J H, Chen J B, Yang J
机构信息
Department of Preventive Veterinary Science, Quartermaster University of PLA, Changchun 130062.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2001;19(2):100-2.
OBJECTIVE
To analyse the genetic heterogeneity of 8 isolates of Cryptosporidium belonging to three species (Cryptosporiduim muris, Cryptosporiduim parvum and Cryptosporidium meleagridis) using RAPD technique.
METHODS
DNA extracts of the 8 isolates were amplified by PCR using 26 out of 200 primers. The amplified products were separated by agar electrophoresis followed by staining with ethidium bromide.
RESULTS
RAPD analysis revealed; 1. Obvious differences among species were found, the average genetic distance being 7.037; 2. Certain differences among different isolates of the same species were found, the average genetic distance being 5.162.
CONCLUSION
RAPD could differentiate the common and respective gene fragments of the 8 isolates of Cryptosporidium.
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