Al-Brikan Faten A, Salem Hala S, Beeching Nick, Hilal Nabeel
Department of Medical Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
J Egypt Soc Parasitol. 2008 Aug;38(2):645-58.
A total of 35 Cryptosporidium positive samples were collected from children in Jeddah city. The samples were microscopically examined with Ziehl Neelsen (ZN) and Auramin phenol (AP) staining methods. Cryptosporidium antigen was detected in the faecal samples by using the Cryptosporidium ELISA kit. Cryptosporidium sp. were identified by targeting an 840 bp of the hyper-variable region of the 18S rRNA gene and about 550 of the first domain (N terminal) of the COWP gene. The sub-genotypic identification of C. parvum and C. hominis isolates was done by targeting the sporozoite antigen gp15/45/60 gene. Four sp. were identified; C. hominis 13/35 (37%), C. parvum 15/35 (42.9%), C. meleagridis 1/35(2.9 %), & C. muris 1/35 (2.9 %). One isolate was a mixed infection of C. parvum & C. hominis.
从吉达市儿童中总共采集了35份隐孢子虫阳性样本。采用齐-尼（ZN）染色法和金胺酚（AP）染色法对样本进行显微镜检查。使用隐孢子虫酶联免疫吸附测定（ELISA）试剂盒检测粪便样本中的隐孢子虫抗原。通过靶向18S rRNA基因高变区的840 bp片段和约550 bp的隐孢子虫表面蛋白（COWP）基因第一结构域（N端）来鉴定隐孢子虫种类。通过靶向子孢子抗原gp15/45/60基因对微小隐孢子虫和人隐孢子虫分离株进行亚基因型鉴定。鉴定出4种隐孢子虫；人隐孢子虫13/35（37%），微小隐孢子虫15/35（42.9%），火鸡隐孢子虫1/35（2.9%），鼠隐孢子虫1/35（2.9%）。1份分离株为微小隐孢子虫和人隐孢子虫混合感染。
