Chazov E I, Veselova S P, Krivosheev O G, Isachenkov V A
Probl Endokrinol (Mosk). 1976 Jan-Feb;22(1):33-9.
Autoradiography was applied to the study of the effect of extirpation of the epiphysis in rats on the level of H3-leucine incorporation by the neurons of 8 couples of the nuclei localized in the anterior, middle and posterior portions of the hypothalamus. Experiments were conducted on female animals of the prepubertal and reproductive age. Pinealectomy was combined with binding (a factor intensifying the physiological status of the epiphysis) and continuous illumination (a factor inhibiting the function of this gland). In the blinded prepubertal rats pinealectomy enhanced the H3-leucine incorporation into the supraoptic nucleus by 38%, into the paraventricular--by 26%, into the dorsomedial--by 61%, into the premammillary dorsal--by 58%, into the ventro-medial--by 40%, into the premammillary ventral--by 36%, and into the anterior hypothalamic--by 26%. In mature rats this index exceeded, after pinealectomy, such in the sham-operated animals (control) in the supraoptic nucleus by 48%, in the paraventral--by 40%, in the premamillary ventral--by 80%, in the arcuate--by 48%, in the premammillary dorsal--by 35%, in the ventro-medial--by 29%, and in the dorso-medial--by 26%. Continuous illumination reduced the stimulating influence of pinealectomy on the H3-leucine incorporation into the neurons of the premammillary and the arcuate nuclei, without changing the difference between the experiment and control in the macrocellular nuclei. On the whole, the results obtained permit a conclusion to be drawn on tonic inhibition by the epiphysis of the extensive hypothalamic zones.
应用放射自显影术研究大鼠松果体摘除术对位于下丘脑前、中、后部分的8对核团神经元中H3 - 亮氨酸掺入水平的影响。实验选用青春期前和生殖期的雌性动物。松果体摘除术分别与结合（一种增强松果体生理状态的因素）和持续光照（一种抑制该腺体功能的因素）相结合。在青春期前致盲大鼠中，松果体摘除术后，视上核中H3 - 亮氨酸掺入增加38%，室旁核增加26%，背内侧核增加61%，乳头前背核增加58%，腹内侧核增加40%，乳头前腹核增加36%，下丘脑前部增加26%。在成年大鼠中，松果体摘除术后，视上核中该指标比假手术动物（对照）超出48%，室旁核超出40%，乳头前腹核超出80%，弓状核超出48%，乳头前背核超出35%，腹内侧核超出29%，背内侧核超出26%。持续光照降低了松果体摘除术对乳头前核和弓状核神经元中H3 - 亮氨酸掺入的刺激作用，而大细胞性核团的实验与对照差异未变。总体而言，所得结果可得出结论：松果体对下丘脑广泛区域存在紧张性抑制作用。
