Takino Masahiko, Daishima Shigeki, Yamaguchi Kenji, Nakahara Taketoshi
Yokogawa Analytical Systems Inc., 3-3-11, Niitaka, Yodogawa-ku, Osaka 532-0033, Japan.
Analyst. 2003 Jan;128(1):46-50. doi: 10.1039/b209675g.
A simple, fast and sensitive liquid chromatography-mass spectrometry (LC-MS) method with automated on-line extraction using turbulent flow chromatography (TFC) for the determination of five catechins in human plasma was developed. In this method, after on-line extraction by its injection onto an extractor column at turbulent flow, five catechins were backwashed onto a reversed phase column via on-line column switching and separated chromatographically at a laminar flow of 1 ml min(-1). Using this tandem LC-LC-MS system, the extraction, the separation and the quantitation of five catechins in human plasma could be achieved with satisfactory selectivity and sensitivity. The limit of detection (S/N = 3) ranged from 0.6 to 2 ng ml(-1). The described procedure was very simple and rapid since no off-line sample preparation was required, total analysis time being 18.5 min.
建立了一种简单、快速且灵敏的液相色谱-质谱联用（LC-MS）方法，该方法采用湍流色谱（TFC）进行自动在线萃取，用于测定人血浆中的五种儿茶素。在此方法中，通过在湍流条件下将样品注入萃取柱进行在线萃取后，五种儿茶素通过在线柱切换反冲到反相柱上，并在1 ml min⁻¹的层流条件下进行色谱分离。使用这种串联LC-LC-MS系统，人血浆中五种儿茶素的萃取、分离和定量能够以令人满意的选择性和灵敏度实现。检测限（S/N = 3）范围为0.6至2 ng ml⁻¹。所述方法非常简单快速，因为无需离线样品制备，总分析时间为18.5分钟。
