Sasaki Yoshimasa, Fujisawa Tomohiko, Ogikubo Kaori, Ohzono Tomoko, Ishihara Kanako, Takahashi Toshio
Veterinary National Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Tokura, Kokubunji, Tokyo, Japan.
J Vet Med Sci. 2003 Jan;65(1):129-31. doi: 10.1292/jvms.65.129.
To isolate Campylobacter spp., the feces of healthy cattle, pigs, and broilers were examined between June 1999 and January 2000. Campylobacter lanienae strains were isolated from the feces of healthy pigs, but not from the feces of cattle or broilers. In six C. lanienae isolates, there was only 21-38% DNA-DNA homology to Campylobacter hyointestinalis subsp. lawsonii strain NCTC 12901. Thus, the primary host of C. lanienae is likely to be the pig and C. lanienae appears to be a species distinct from C. hyointestinalis subsp. lawsonii. In addition, an intervening sequence of 226 bp in the 16S rRNA gene was found in four isolates.
为分离弯曲杆菌属细菌,于1999年6月至2000年1月期间对健康牛、猪和肉鸡的粪便进行了检测。从健康猪的粪便中分离出了拉尼尔弯曲杆菌菌株,但未从牛或肉鸡的粪便中分离到。在6株拉尼尔弯曲杆菌分离株中,与劳森氏猪肠弯曲杆菌亚种NCTC 12901菌株的DNA-DNA同源性仅为21%-38%。因此,拉尼尔弯曲杆菌的主要宿主可能是猪,且拉尼尔弯曲杆菌似乎是一个与劳森氏猪肠弯曲杆菌亚种不同的物种。此外,在4株分离株中发现16S rRNA基因中有一段226 bp的间隔序列。