影响哈茨木霉1073 D3产木聚糖酶的一些生理因素的测定

Determination of some physiological factors affecting xylanase production from Trichoderma harzianum 1073 D3.

作者信息

Seyis I, Aksoz N

机构信息

Hacettepe University, Faculty of Science, Department of Biology, Beytepe, 06532 Ankara, Turkey.

出版信息

New Microbiol. 2003 Jan;26(1):75-81.

PMID:12578314

Abstract

In this study, different Trichoderma strains were tested and Trichoderma harzianum 1073 D3 was found to be the most potent xylanase producer. Then some cultural parameters, namely, incubation time, substrate concentration, initial culture pH and temperature were optimized in order to increase xylanase production from Trichoderma harzianum 1073 D3. The optimum incubation time was found to be 13 days. It was concluded that 1% xylan concentration is suitable for high xylanase production rate. The optimum temperature and pH were found to be 30 degrees C and 7, respectively. Also, it was determined that agitation during growth was suitable for efficient production.

摘要

在本研究中，对不同的木霉菌株进行了测试，发现哈茨木霉1073 D3是最有效的木聚糖酶生产者。然后，为了提高哈茨木霉1073 D3的木聚糖酶产量，对一些培养参数，即培养时间、底物浓度、初始培养pH值和温度进行了优化。发现最佳培养时间为13天。得出结论，1%的木聚糖浓度适合高木聚糖酶生产率。发现最佳温度和pH值分别为30℃和7。此外，还确定生长期间的搅拌有利于高效生产。