Park Y S, Kang S W, Lee J S, Hong S I, Kim S W
Graduate School of Biotechnology, Korea University, 1, Anam-dong, Sungbuk-ku, Seoul, 136-701, Korea.
Appl Microbiol Biotechnol. 2002 May;58(6):761-6. doi: 10.1007/s00253-002-0965-0. Epub 2002 Mar 20.
The initial moisture content, cultivation time, inoculum size and concentration of basal medium were optimized in solid state fermentation (SSF) for the production of xylanase by an Aspergillus niger mutant using statistical experimental designs. The cultivation time and concentration of basal medium were the most important factors affecting xylanase activity. An inoculum size of 5 x 10(5) spores/g, initial moisture content of 65%, cultivation time of 5 days and 10 times concentration of basal medium containing 50 times concentration of corn steep liquor were optimum for xylanase production in SSF. Under the optimized conditions, the activity and productivity of xylanase obtained after 5 days of fermentation were 5,071 IU/g of rice straw and 14,790 IU l(-1) h(-1), respectively. The xylanase activity predicted by a polynomial model was 5,484 IU/g of rice straw.
采用统计实验设计，对黑曲霉突变株固态发酵（SSF）生产木聚糖酶的初始水分含量、培养时间、接种量和基础培养基浓度进行了优化。培养时间和基础培养基浓度是影响木聚糖酶活性的最重要因素。接种量为5×10⁵个孢子/克、初始水分含量为65%、培养时间为5天以及基础培养基浓度为含50倍玉米浆浓度的10倍，这些条件对固态发酵生产木聚糖酶最为适宜。在优化条件下，发酵5天后获得的木聚糖酶活性和生产率分别为5071 IU/克稻草和14790 IU l⁻¹ h⁻¹。多项式模型预测的木聚糖酶活性为5484 IU/克稻草。
