Hu Ying, Zhang Li-Yan, Ma Guan-Jie, Jiang Xue-Ying, Zhao Chun-Hua
State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2001 Dec;9(4):289-293.
There has been an increasing interest in recent years in the stromal cell system. The stroma is a complex tissue, composed of a number vascular and connective tissue cell types including endothelial cells, adipocytes, smooth muscle cells, osteogenic cells, and stromal cells. The marrow mesenchymal stem cells are capable of self-renewal and differentiate into various connective tissue lineages including bone, cartilage, tendon, muscle, fat, and marrow stroma. Recently, techniques for the isolation of adult bone marrow-derived human and animal mesenchymal stem cells have been described, as well as the methods to directing their differentiation into osteogenic, chondrogenic, tendogenic, myogenic, adipogenic, and marrow stromal lineages. But there is no report about the fetal bone marrow- and liver-derived mesenchymal stem cells. Are they the same or not? In our assay, human fetal mesenchymal stem cells from 4 - 5 months old human fetal bone marrow and liver low-density mononuclear cells were cultured, and the cell cycle, immunophenotype and ex vivo expansion properties were studied. Results showed that the mesenchymal stem cells from fetal liver and fetal bone marrow were similar in morphology, growth character and immuno-phenotypes, but the liver mesenchymal cells manifested higher ability to support hematopoiesis than the marrow-derived mesenchymal cells. This study demonstrates that adherent fetal marrow- and liver-derived cells cultured in the absence of differentiation stimulus gave rise to a population of cells with phenotypical features of mesenchymal stem cells, and should be enough to sustain a steady supply of low differentiated cells upon proliferation.
近年来，人们对基质细胞系统的兴趣与日俱增。基质是一种复杂的组织，由多种血管和结缔组织细胞类型组成，包括内皮细胞、脂肪细胞、平滑肌细胞、成骨细胞和基质细胞。骨髓间充质干细胞能够自我更新，并分化为各种结缔组织谱系，包括骨、软骨、肌腱、肌肉、脂肪和骨髓基质。最近，已经描述了分离成人骨髓来源的人和动物间充质干细胞的技术，以及指导它们分化为成骨、软骨、肌腱、肌、脂肪和骨髓基质谱系的方法。但是，关于胎儿骨髓和肝脏来源的间充质干细胞尚无报道。它们是否相同呢？在我们的实验中，培养了来自4 - 5个月大的人类胎儿骨髓和肝脏低密度单核细胞的人类胎儿间充质干细胞，并研究了细胞周期、免疫表型和体外扩增特性。结果表明，胎儿肝脏和胎儿骨髓来源的间充质干细胞在形态、生长特性和免疫表型方面相似，但肝脏间充质细胞比骨髓来源的间充质细胞表现出更高的支持造血能力。本研究表明，在无分化刺激的情况下培养的贴壁胎儿骨髓和肝脏来源的细胞产生了一群具有间充质干细胞表型特征的细胞，并且在增殖时应该足以维持低分化细胞的稳定供应。
