超临界流体萃取-毛细管气相色谱法测定丹参中丹参酮的含量
[SFE-CGC determination of tanshinone in Salvia miltiorrhiza Bunge].
作者信息
Chen B, Zhu M, Xing W X, Liu L L, Wu Y T
机构信息
School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
出版信息
Yao Xue Xue Bao. 2001 Jan;36(1):55-7.
AIM
To analyze tanshinone IIA in Salvia miltiorrhiza Bunge by supercritical fluid extraction (SFE) coupled with capillary gas chromatography (CGC).
METHODS
The solution pressure was predicted by solubility parameter theory, the extraction conditions were confirmed by orthogonal design in which temperature, volume of modifier and dynamic extracting volume had been studied in three levels.
RESULTS
This method is quick, convenient, precise and accurate, added sample recovery is 95.3%, RSD = 4.27%.
CONCLUSION
Compared with the method of ultrasonic extraction, significantly better qualitative and quantitative result could be obtained by SFE-CGC and expend less organic solvent then make less pollution.
目的
采用超临界流体萃取（SFE）结合毛细管气相色谱法（CGC）分析丹参中的丹参酮IIA。
方法
用溶解度参数理论预测溶液压力，通过正交设计确定萃取条件，考察了温度、改性剂体积和动态萃取体积三个水平。
结果
该方法快速、简便、准确，加样回收率为95.3%，相对标准偏差（RSD）为4.27%。
结论
与超声提取法相比，超临界流体萃取-毛细管气相色谱法（SFE-CGC）能获得更好的定性和定量结果，且有机溶剂消耗少，污染小。
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