Wu Wei-hong, Wang Ning, Cai Shao-song, Wei Hong
ShanDong Medical College, Jinan 250002, China.
Zhong Yao Cai. 2009 Jan;32(1):118-21.
To explore a method on-line determining the contents of tanshinone II A in returning extraction process of Salvia miltiorrhiza by AOTF-Near infrared spectroscopy.
Firstly, the sample was collected in the extraction process of Salvia miltiorrhiza. Then HPLC was used as a reference method to determine the contents of tanshinone II A in the sample. Multivariate calibration models based on PLS1 algorithm were developed to correlate the spectra and the corresponding values determined by the reference methods.
RMSECV of the models for tanshinone II A was 0.0092. The correlation coefficients of the calibration models was 0.9918. External validation with external validation samples proved that the relative deviation was 5.74%.
AOTF-NIRS can be used in the determination of tanshinone II A in returning extraction process of Salvia miltiorrhiza. The method is rapid, accurate and non-destructive, and can be applied for process analysis and quality control of Chinese medicine manufacturing process.
探索一种利用声光可调滤光器 - 近红外光谱法在线测定丹参回流提取过程中丹参酮ⅡA含量的方法。
首先，在丹参提取过程中采集样品。然后，以高效液相色谱法作为参考方法测定样品中丹参酮ⅡA的含量。基于偏最小二乘法1（PLS1）算法建立多元校正模型，将光谱与参考方法测定的相应值进行关联。
丹参酮ⅡA模型的交叉验证均方根误差（RMSECV）为0.0092。校正模型的相关系数为0.9918。用外部验证样品进行外部验证，结果表明相对偏差为5.74%。
声光可调滤光器 - 近红外光谱法可用于丹参回流提取过程中丹参酮ⅡA的测定。该方法快速、准确、无损，可应用于中药生产过程的过程分析和质量控制。
