Dasgupta Amitava, Wu Sang, Actor Jeffrey, Olsen Margaret, Wells Alice, Datta Pradip
Department of Pathology and Laboratory Medicine, University of Texas-Houston Medical School, 6431 Fannin, MSB 2.292, Houston, TX 77030, USA.
Am J Clin Pathol. 2003 Feb;119(2):298-303. doi: 10.1309/34BJ-ECP7-UK6F-H13V.
Asian and Siberian ginsengs contain glycosides with structural similarities to digoxin. We studied potential interference of ginseng in 5 digoxin immunoassays in 3 Asian (2 liquid extracts, 1 capsule) and 3 Siberian ginseng preparations (1 liquid extract, 2 capsules). With the fluorescence polarization immunoassay (FPIA), we observed apparent digoxin activity in 1 Asian liquid preparation and in the liquid extract and 1 capsule form of Siberian ginseng. In mice fed ginseng, we observed digoxin activities in the serum (Asian, 0.48-0.68 ng/mL [0.6-0.9 nmol/L]; Siberian, 0.20-0.47 ng/mL [0.3-0.6 nmol/L]), indicating that such interferences also occur in vivo. Serum pools prepared from samples from patients receiving digoxin and then supplemented with Asian or Siberian ginseng showed falsely increased digoxin values using the FPIA (e.g., for Asian ginseng, 1.54 ng/mL [2.0 nmol/L] vs control value, 1.10 ng/mL [1.4 nmol/L]) and falsely decreased values using the microparticle enzyme immunoassay (MEIA; 0.73 ng/mL [0.9 nmol/L] vs control value, 1.04 ng/mL [1.3 nmol/L]). Digoxin-like immunoreactive substances (DLISs) showed synergistic effects with ginsengs in interfering with the FPIA and MEIA for digoxin. No interference was observed with 3 other digoxin assays, even in the presence of elevated DLISs.
亚洲人参和西伯利亚人参含有结构与地高辛相似的糖苷。我们研究了3种亚洲人参制剂（2种液体提取物、1种胶囊）和3种西伯利亚人参制剂（1种液体提取物、2种胶囊）对5种地高辛免疫测定法的潜在干扰。采用荧光偏振免疫测定法（FPIA），我们在1种亚洲人参液体制剂以及西伯利亚人参的液体提取物和1种胶囊制剂中观察到了明显的地高辛活性。在喂食人参的小鼠中，我们在血清中观察到了地高辛活性（亚洲人参，0.48 - 0.68 ng/mL [0.6 - 0.9 nmol/L]；西伯利亚人参，0.20 - 0.47 ng/mL [0.3 - 0.6 nmol/L]），表明这种干扰在体内也会发生。从接受地高辛治疗的患者样本中制备血清池，然后添加亚洲或西伯利亚人参，使用FPIA时地高辛值出现假性升高（例如，对于亚洲人参，1.54 ng/mL [2.0 nmol/L] 对比对照值1.10 ng/mL [1.4 nmol/L]），而使用微粒酶免疫测定法（MEIA）时地高辛值出现假性降低（0.73 ng/mL [0.9 nmol/L] 对比对照值1.04 ng/mL [1.3 nmol/L]）。类地高辛免疫反应性物质（DLISs）在干扰地高辛的FPIA和MEIA方面与人参具有协同作用。即使存在升高的DLISs，其他3种地高辛测定法也未观察到干扰。
