[异烟肼的流动注射双安培分析]
[Flow injection biamperometric analysis of isoniazid].
作者信息
Zhang J C, Zhao C, Song J F
机构信息
Institute of Analytical Science, Northwest University, Xi'an 710069, China.
出版信息
Yao Xue Xue Bao. 2001 Sep;36(9):679-82.
AIM
To establish a simple, rapid, and accurate electrochemical method for on-line determination of isoniazid.
METHODS
Based on the flow injection biamperometry for irreversible couple system, and using two preanodized platinum electrodes with the applied potential difference of 0 V, the biamperometric method for the determination of isoniazid has been proposed by coupling the catalytic oxidation of isoniazid and the reduction of platinum oxide.
RESULTS
Common excipients, inorganic ions, amino acids, vitamins and proteins do not interfere with the determination. Linear relationship between current and the concentration of isoniazid is obtained in the range of 1.0 x 10(-6)-1.0 x 10(-4) mol.L-1 (gamma = 0.998, n = 11). The RSD of 1.8% was obtained for 8 successive determinations of 1.0 x 10(-5) mol.L-1 isoniazid.
CONCLUSION
The proposed method has been shown to be sensitive, selective, rapid (120 samples.h-1), and suitable for the on-line direct determination of isoniazid.
目的
建立一种简单、快速、准确的在线测定异烟肼的电化学方法。
方法
基于不可逆偶合体系的流动注射双安培法，采用两个预阳极氧化铂电极，施加电位差为0 V，通过异烟肼的催化氧化与氧化铂的还原相偶合，提出了测定异烟肼的双安培法。
结果
常见辅料、无机离子、氨基酸、维生素和蛋白质不干扰测定。异烟肼浓度在1.0×10⁻⁶ - 1.0×10⁻⁴ mol·L⁻¹范围内，电流与浓度呈线性关系（γ = 0.998，n = 11）。对1.0×10⁻⁵ mol·L⁻¹异烟肼连续8次测定，相对标准偏差为1.8%。
结论
所提出的方法灵敏、选择性好、快速（120个样品·小时⁻¹），适用于异烟肼的在线直接测定。
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