Bax抑制剂的过表达可抑制真菌激发子诱导的水稻（Oryza sativa L）细胞死亡。

Overexpression of Bax inhibitor suppresses the fungal elicitor-induced cell death in rice (Oryza sativa L) cells.

作者信息

Matsumura H, Nirasawa S, Kiba A, Urasaki N, Saitoh H, Ito M, Kawai-Yamada M, Uchimiya H, Terauchi R

机构信息

Iwate Biotechnology Research Center, Kitakami, Iwate 024-0003, Japan.

出版信息

Plant J. 2003 Feb;33(3):425-34. doi: 10.1046/j.1365-313x.2003.01639.x.

DOI:10.1046/j.1365-313x.2003.01639.x

PMID:12581301

Abstract

Treatment of suspension-cultured cells of rice (Oryza sativa L.) with cell wall extract of rice blast fungus (Magnaporthe grisea) elicits a rapid generation of H2O2, alkalinization of culture medium, and eventual cell death. To elucidate genes involved in these processes, we exploited SAGE (Serial Analysis of Gene Expression) technique for the molecular analysis of cell death in suspension-cultured cells treated with the elicitor. Among the downregulated genes in the elicitor-treated cells, a BI-1 gene coding for Bax inhibitor was identified. Transgenic rice cells overexpressing Arabidopsis BI-1 gene showed sustainable cell survival when challenged with M. grisea elicitor. Thus, the plant Bax inhibitor plays a functional role in regulating cell death in the rice cell culture system.

摘要

用稻瘟病菌（Magnaporthe grisea）的细胞壁提取物处理水稻（Oryza sativa L.）悬浮培养细胞，会迅速产生过氧化氢、培养基碱化，并最终导致细胞死亡。为了阐明参与这些过程的基因，我们利用基因表达系列分析（SAGE）技术对用激发子处理的悬浮培养细胞中的细胞死亡进行分子分析。在激发子处理的细胞中下调的基因中，鉴定出一个编码Bax抑制剂的BI-1基因。过表达拟南芥BI-1基因的转基因水稻细胞在用稻瘟病菌激发子处理时表现出可持续的细胞存活。因此，植物Bax抑制剂在水稻细胞培养系统中调节细胞死亡方面发挥着功能性作用。