激发子诱导基因OsWRKY53参与水稻中防御相关基因的表达。
Involvement of the elicitor-induced gene OsWRKY53 in the expression of defense-related genes in rice.
作者信息
Chujo Tetsuya, Takai Ryota, Akimoto-Tomiyama Chiharu, Ando Sugihiro, Minami Eiichi, Nagamura Yoshiaki, Kaku Hanae, Shibuya Naoto, Yasuda Michiko, Nakashita Hideo, Umemura Kenji, Okada Atsushi, Okada Kazunori, Nojiri Hideaki, Yamane Hisakazu
机构信息
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
出版信息
Biochim Biophys Acta. 2007 Jul-Aug;1769(7-8):497-505. doi: 10.1016/j.bbaexp.2007.04.006. Epub 2007 Apr 22.
We present a detailed characterization of the chitin oligosaccharide elicitor-induced gene OsWRKY53. OsWRKY53 was also induced in suspension-cultured rice cells by a fungal cerebroside elicitor and in rice plants by infection with the blast fungus Magnaporthe grisea. A fusion of OsWRKY53 with green fluorescent protein was detected exclusively in the nuclei of onion epidermal cells, and OsWRKY53 protein specifically bound to W-box elements. A transient assay using the particle bombardment method showed that OsWRKY53 is a transcriptional activator. A microarray analysis revealed that several defense-related genes, including pathogenesis-related protein genes such as PBZ1, were upregulated in rice cells overexpressing OsWRKY53. Finally, overexpression of OsWRKY53 in rice plants resulted in enhanced resistance to M. grisea. These results strongly suggest that OsWRKY53 is a transcription factor that plays important roles in elicitor-induced defense signaling pathways in rice.
我们展示了几丁质寡糖激发子诱导基因OsWRKY53的详细特征。真菌脑苷脂激发子也能在悬浮培养的水稻细胞中诱导OsWRKY53表达，稻瘟病菌Magnaporthe grisea侵染水稻植株时也会诱导该基因表达。在洋葱表皮细胞的细胞核中仅检测到绿色荧光蛋白与OsWRKY53的融合蛋白，并且OsWRKY53蛋白能特异性结合W-盒元件。利用粒子轰击法进行的瞬时分析表明OsWRKY53是一种转录激活因子。微阵列分析显示，在过表达OsWRKY53的水稻细胞中，包括病程相关蛋白基因如PBZ1在内的几个防御相关基因上调。最后，在水稻植株中过表达OsWRKY53导致对稻瘟病菌的抗性增强。这些结果有力地表明，OsWRKY53是一种转录因子，在水稻激发子诱导的防御信号通路中发挥重要作用。
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