非那雄胺的极谱行为及测定

Polarographic behavior and determination of finasteride.

作者信息

Amer Sawsan M

机构信息

Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El Eini Street, 11562 Cairo, Egypt.

出版信息

Farmaco. 2003 Feb;58(2):159-63. doi: 10.1016/s0014-827x(02)00015-0.

DOI:10.1016/s0014-827x(02)00015-0

PMID:12581782

Abstract

The polarographic behavior of finasteride at the dropping mercury electrode (DME) was studied adopting direct current (DC(t)), alternating current (AC(t)) and differential-pulse polarography (DPP) modes. In Britton-Robinson buffer (BRb), finasteride exhibited cathodic waves over the pH range 6-12. At pH 10, a well-defined cathodic wave was obtained. The latter could be characterized as being irreversible, diffusion-controlled and partially affected by adsorption phenomenon. The number of electrons involved in the reduction process was accomplished and a proposal of the electrode reaction was presented. The current-concentration plots were rectilinear over the ranges 8-40 and 2-30 microg ml(-1) using DC(t) and DPP modes, respectively. The minimum delectability was 0.2 microg ml(-1) (5.4 x 10(-7) M), for the latter. The proposed method was successfully applied to the determination of finasteride in its commercial capsules and the results obtained were in good agreement with those given with a reference method.

摘要

采用直流极谱法（DC(t)）、交流极谱法（AC(t)）和微分脉冲极谱法（DPP）研究了非那雄胺在滴汞电极（DME）上的极谱行为。在 Britton-Robinson 缓冲液（BRb）中，非那雄胺在 pH 6 - 12 范围内呈现阴极波。在 pH 10 时，可获得明确的阴极波。该阴极波的特征为不可逆、受扩散控制且部分受吸附现象影响。确定了还原过程中涉及的电子数，并提出了电极反应的设想。分别使用 DC(t) 和 DPP 模式时，电流 - 浓度曲线在 8 - 40 和 2 - 30 μg ml⁻¹ 范围内呈线性。后者的最低检测限为 0.2 μg ml⁻¹（5.4×10⁻⁷ M）。所提出的方法成功应用于测定非那雄胺商业胶囊中的含量，所得结果与参考方法的结果高度一致。