[大鼠肌肉卫星细胞在周期性机械拉伸下三维培养的形态学研究]
[Morphological study on three-dimensional culture of rat muscle satellite cells under cyclic mechanical stretch].
作者信息
Xiong Shao-hu, Li Ning, Zhao Jin-mei, Yu Lei, Liao Hua, Qin Ting-wu, Zhong Shi-zhen
机构信息
Key Laboratory of Tissue Construction and Detection of Guangdong Province, First Military Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2003 Feb;23(2):141-3.
OBJECTIVE
To study the effect of mechanical stretch on the morphologic change of rat muscle satellite cell in the three-dimensional culture.
METHODS
Based on the apparatus for three-dimensional cell cultures under a cyclic mechanical strain, a specific stretch pattern (10% elongation, 10 stretches/min for 10 min of each hour 48 h total) was applied to cell-scaffolding composites.
RESULTS
Under the stretch pattern, the shape of satellite cells changed to oblate and spread to the direction of the stretch. Furthermore, the myotube was observed. On the contrary, the satellite cells spread to the all direction, and the formation of the myotube was not been found in the control group.
CONCLUSION
Cyclic mechanical stretch is helpful for the formation of the ideal directivity and these results are compatible with a significant role for the stretch in tissue-engineered muscle construction.
目的
研究机械拉伸对三维培养的大鼠肌肉卫星细胞形态变化的影响。
方法
基于循环机械应变下的三维细胞培养装置，对细胞-支架复合材料施加特定的拉伸模式（伸长10%，每分钟10次拉伸，每小时10分钟，共48小时）。
结果
在该拉伸模式下，卫星细胞的形状变为扁圆形并向拉伸方向伸展。此外，观察到了肌管。相反，在对照组中，卫星细胞向各个方向伸展，未发现肌管形成。
结论
循环机械拉伸有助于形成理想的方向性，这些结果与拉伸在组织工程肌肉构建中的重要作用相符。
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