Suppr超能文献

Study of protein-protein interactions by fluorescence of tryptophan analogs: application to immunoglobulin G binding domain of streptococcal protein G.

作者信息

Li Qi, Du Hai-Ning, Hu Hong-Yu

机构信息

Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, People's Republic of China.

出版信息

Biopolymers. 2003;72(2):116-22. doi: 10.1002/bip.10300.

Abstract

The protein-protein interaction system often contains many fluorophores that may significantly interfere with the quantitative determination of the binding abilities. To solve this perplexing problem, we biosynthetically incorporated the two tryptophan analogs, 5-hydroxytryptophan and 7-azatryptophan, into the immunoglobulin G (IgG) binding domain of streptococcal protein G (PGBD). The exclusive excitation and novel fluorescence changes in both the intensity and anisotropy are beneficial to reporting the details of the interactions between PGBD and the IgG fragments and enable assessment of the binding abilities. The dissociation constants are estimated to be 0.28 microM for the binding of human Fc and 8.0 microM for mouse Fc. The results clearly demonstrate that labeling of tryptophan analogs has very little effect on the binding abilities and is broadly applicable to quantitatively studying protein-protein interactions in a whole biomolecular complex.

摘要

相似文献

3
Protein-protein interaction regulates proteins' mechanical stability.
J Mol Biol. 2008 May 16;378(5):1132-41. doi: 10.1016/j.jmb.2008.03.046. Epub 2008 Mar 28.
6
Metal-linked dimerization in the iron-dependent regulator from Mycobacterium tuberculosis.
Biochemistry. 2006 Oct 17;45(41):12480-90. doi: 10.1021/bi060797s.

引用本文的文献

1
A functional single-molecule binding assay via force spectroscopy.
Proc Natl Acad Sci U S A. 2007 Oct 2;104(40):15677-81. doi: 10.1073/pnas.0705367104. Epub 2007 Sep 25.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验