构建功能性 IgG 结合荧光素酶融合蛋白，用于快速检测特定的细菌菌株。

Construction of a functional IgG-binding luciferase fusion protein for the rapid detection of specific bacterial strains.

机构信息

Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan.

出版信息

Analyst. 2011 Jan 7;136(1):71-2. doi: 10.1039/c0an00460j. Epub 2010 Oct 20.

DOI:10.1039/c0an00460j

PMID:20959930

Abstract

A fusion protein consisting of two IgG-binding domains of streptococcal protein G and firefly luciferase was constructed, and a simple and specific bioluminescent immunodetection system for bacterial strains was developed.

摘要

构建了由链球菌蛋白 G 的两个 IgG 结合结构域和萤火虫荧光素酶组成的融合蛋白，并开发了一种简单而特异的用于检测细菌株的生物发光免疫检测系统。