益母草生物活性测定的研究（一）——标准子宫模型的建立及子宫环境条件的优化
[Study on the biological assay of Herba Leonuri(I)--Establishment of standard uterus models and optimizing uterus environment conditions].
作者信息
Yang Minghua, Yang Subei, Jin Zuhan, Guo Yuefang
机构信息
Zhejiang Provincial Institute of Chinese Materia Medica, Hangzhou 310023.
出版信息
Zhong Yao Cai. 2002 May;25(5):333-6.
OBJECTIVE
To study the contracting uterus effect of Herba Leonuri, establish the standard uterus models, and optimize the environment conditions which could influence Herba Leonuri actions on uterus.
METHODS
By comparing the contracting uterus effect of Herba Leonuri in mice, rats and rabbits, the standard uterus models was established. The optimum extra conditions of uterus were selected by orthogonal design methods.
RESULTS
The isolated rat uterus pre-oestrus was selected for the standard uterus, the optimum uterus environment conditions as follows: the pre-burden of uterus was 1.0 g, the temperature of uterus nutrition solution is 32 degrees C, the assay of CaCl2 and NaHCO3 per 1000 ml nutrition solution was respectively 0.06 g and 0.25 g.
CONCLUSION
Under the selected conditions, the contracting uterus effect of Herba Leonuri were more obvious and the errors of bioassay were less smaller.
目的
研究益母草对子宫的收缩作用，建立标准子宫模型，并优化可能影响益母草子宫作用的环境条件。
方法
通过比较益母草对小鼠、大鼠和家兔子宫的收缩作用，建立标准子宫模型。采用正交设计方法选择子宫的最佳额外条件。
结果
选择动情前期的离体大鼠子宫作为标准子宫，最佳子宫环境条件如下：子宫前负荷为1.0 g，子宫营养液温度为32℃，每1000 ml营养液中氯化钙和碳酸氢钠的含量分别为0.06 g和0.25 g。
结论
在所选条件下，益母草对子宫的收缩作用更明显，生物测定误差更小。
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