益母草生物测定研究——分析益母草与缩宫素的剂量反应曲线并建立适当的效价模式
[Study on the biological assay of Herba Leonuri--analysis the dosage response curve of Herba Leonuri and oxytocin and establishment of adequate potency pattern].
作者信息
Yang Minghua, Yang Subei, Jin Zuhan, Zhu Shuangyou
机构信息
Zhejiang Provincial Institute of Chinese Medicine, Hangzhou 310023.
出版信息
Zhong Yao Cai. 2002 Jun;25(6):409-11.
OBJECTIVE
To establish the effective patten of Herba Leonuri to be suitable for biological assay.
METHODS
By comparing the dosage-effect curve of Herba Leonuri and oxytocin the suitable effective pattern was set up.
RESULTS
The experiment suggest that the uterus contract height from 20 mm to 85 mm is the adequate effective range. In this range, the effect both Herba Leonuri and oxytocin should plot as a straight line against the log dose and two lines are basically parallel.
CONCLUSION
In the light of establishing effective pattern, the biological assay of Herba Leonuri may pattern the method of oxytocin biological assay to regard oxytocin as standard. The result error of Herba Leonuri assay is under 20%, not exceed the demand limits of biological assay.
目的
建立适合生物测定的益母草有效模式。
方法
通过比较益母草与缩宫素的量效曲线来建立合适的有效模式。
结果
实验表明子宫收缩高度从20毫米至85毫米为适宜的有效范围。在此范围内，益母草和缩宫素的效应与对数剂量作图均应为直线，且两直线基本平行。
结论
从建立有效模式来看，益母草的生物测定可仿照缩宫素生物测定方法，以缩宫素为标准。益母草测定的结果误差在20%以内，不超过生物测定的要求限度。
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