Endsley Janice J, Quade Mark J, Terhaar Brett, Roth James A
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
Vet Ther. 2002 Winter;3(4):364-72.
Expression of CD25 (interleukin-2 receptor alpha chain) was used to monitor antigen-specific activation of T lymphocyte subsets (CD4+, CD8+, and gamma delta T cells) from cattle immunized with modified-live virus (MLV) bovine viral diarrhea virus (BVDV) vaccines. Two groups of 15 animals each were vaccinated with one dose of either BVDV genotype 1 (BVDV-1) or BVDV-1 and BVDV genotype 2 (BVDV-1/2). Six animals negative for both BVDV antibody and BVDV virus were used as negative controls. Three animals vaccinated 7 and 5 weeks before the start of the experiment with MLV BVDV-1 vaccine served as positive controls. Blood samples were taken from the negative control group, the positive control group, and the BVDV-1/2 group 0, 21, 35, 60, and 90 days after vaccination. Blood samples were taken from the BVDV-1 group 0, 21, and 90 days after vaccination. Isolated peripheral blood lymphocytes from immunized and control animals were incubated for 5 days with and without BVDV-1 or BVDV-2. Compared with nonvaccinated animals, a significant (P <.05) increase in expression of CD25 by CD4+ (60 days), CD8+, and gammadelta T (35 to 90 days) lymphocytes from the group given BVDV-1/2 was detected following in vitro exposure to BVDV-1 or BVDV-2 after vaccination. The CD8+ and gammadelta T cells from the group vaccinated with BVDV-1 had significantly (P <.05) increased expression of CD25 compared with nonvaccinates following postvaccination exposure to in vitro BVDV-1 but not to BVDV-2. There was no significant difference between the two vaccinated groups in CD25 expression on any of the T cell subsets in response to BVDV-1 or BVDV-2 exposure. A single administration of MLV BVDV vaccine may be more effective at stimulating CD8+ and gammadelta T cell-specific immune responses to the homologous genotype than to the heterologous genotype.
利用CD25（白细胞介素-2受体α链）的表达来监测用改良活病毒（MLV）牛病毒性腹泻病毒（BVDV）疫苗免疫的牛的T淋巴细胞亚群（CD4 +、CD8 +和γδT细胞）的抗原特异性激活。两组，每组15只动物，分别接种一剂BVDV基因型1（BVDV-1）或BVDV-1和BVDV基因型2（BVDV-1/2）。6只BVDV抗体和BVDV病毒均为阴性的动物用作阴性对照。3只在实验开始前7周和5周用MLV BVDV-1疫苗接种的动物用作阳性对照。在接种疫苗后0、21、35、60和90天，从阴性对照组、阳性对照组和BVDV-1/2组采集血样。在接种疫苗后0、21和90天，从BVDV-1组采集血样。将免疫动物和对照动物分离的外周血淋巴细胞在有和没有BVDV-1或BVDV-2的情况下孵育5天。与未接种疫苗的动物相比，接种BVDV-1/2组的CD4 +（60天）、CD8 +和γδT（35至90天）淋巴细胞在接种疫苗后体外暴露于BVDV-1或BVDV-2后，CD25表达显著（P <.05）增加。接种BVDV-1组的CD8 +和γδT细胞在接种疫苗后体外暴露于BVDV-1而非BVDV-2后，与未接种疫苗的动物相比，CD25表达显著（P <.05）增加。在暴露于BVDV-1或BVDV-2时，两个接种组在任何T细胞亚群上的CD25表达没有显著差异。单次接种MLV BVDV疫苗在刺激CD8 +和γδT细胞对同源基因型而非异源基因型的特异性免疫反应方面可能更有效。
