Platt Ratree, Widel Philip W, Kesl Lyle D, Roth James A
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States.
Vaccine. 2009 Jul 16;27(33):4508-19. doi: 10.1016/j.vaccine.2009.05.012. Epub 2009 May 27.
The aim of this study was to evaluate the ability of a pentavalent (BVDV types 1 and 2, BHV-1, BRSV, and PI-3) modified live virus (MLV) vaccine given to 1-2-, 4-5-, and 7-8-week-old calves with maternal antibodies to induce humoral and cellular immune responses and protect calves from virulent BVDV type 2. Eight calves in each age group were vaccinated and four served as controls. All calves were challenged intranasally with BVDV type 2, 12 weeks after vaccination. SVN titers to all five viruses declined in all groups after vaccination (except 4-5-week-old calves to BVDV type 1). After challenge, the SVN titers for both types of BVDV showed anamnestic responses in calves vaccinated at 4-5 and 7-8 weeks, but not at 1-2 weeks of age. In all groups, T cell subsets responded specifically to BVDV types 1 and 2 but not to BHV-1, BRSV, or PI-3 after vaccination by increasing their expression of activation markers (CD25, IFN-gamma and IL-4). All vaccinated calves were significantly protected from BVDV type 2 challenge.
本研究的目的是评估一种五价（1型和2型牛病毒性腹泻病毒、牛疱疹病毒1型、牛呼吸道合胞病毒和3型副流感病毒）改良活病毒（MLV）疫苗对具有母源抗体的1 - 2周龄、4 - 5周龄和7 - 8周龄犊牛诱导体液免疫和细胞免疫反应以及保护犊牛免受2型强毒力牛病毒性腹泻病毒感染的能力。每个年龄组的8头犊牛接种疫苗，4头作为对照。所有犊牛在接种疫苗12周后经鼻内接种2型牛病毒性腹泻病毒。接种疫苗后所有组中针对所有五种病毒的血清中和（SVN）效价均下降（4 - 5周龄犊牛针对1型牛病毒性腹泻病毒除外）。攻毒后，4 - 5周龄和7 - 8周龄接种疫苗的犊牛中两种类型牛病毒性腹泻病毒的SVN效价出现回忆反应，但1 - 2周龄时未出现。在所有组中，接种疫苗后T细胞亚群通过增加其激活标志物（CD25、γ干扰素和白细胞介素-4）的表达对1型和2型牛病毒性腹泻病毒产生特异性反应，但对牛疱疹病毒1型、牛呼吸道合胞病毒或3型副流感病毒无反应。所有接种疫苗的犊牛均受到显著保护，免受2型牛病毒性腹泻病毒的攻毒。
