Soussan Patrick, Tuveri Rosella, Nalpas Bertrand, Garreau Florianne, Zavala Flora, Masson Annie, Pol Stanislas, Brechot Christian, Kremsdorf Dina
INSERM U370, CHU Necker and Institut Pasteur, 156 rue de Vaugirard, 75015 Paris, France.
J Hepatol. 2003 Mar;38(3):343-8. doi: 10.1016/s0168-8278(02)00422-1.
BACKGROUND/AIMS: We have previously demonstrated the in vivo expression of a new spliced hepatitis B virus (HBV) protein (HBSP) encoded by a singly spliced pregenomic RNA. The present study was designed to evaluate the impact of HBSP expression on the clinical status and liver pathology of HBV infection.
Sera from 125 chronic HBV carriers were tested for the presence of HBSP antibodies by an indirect enzyme-linked immunosorbent assay test. The severity of liver damage was evaluated using the Knodell score.
Anti-HBSP antibody prevalence in HBV chronic carriers was 46%. We highlighted the concomitant expression of HBSP protein and anti-HBSP antibody. An association between anti-HBSP antibody detection and serum markers of HBV replication was demonstrated. With respect to HBV-related liver disease, an association was only observed with the severity of fibrosis. Furthermore, an elevation of secreted tumor necrosis factor alpha (TNFalpha), but not of soluble TNFalpha receptor 75, was observed in anti-HBSP-antibody-positive patients. Multivariate analysis showed that anti-HBSP antibody detection was independently associated with viral replication, severity of fibrosis and elevated TNFalpha secretion.
Our data suggest the hypothesis that HBSP might play a role in the natural history of HBV infection and may be involved in the pathogenesis and/or persistence of HBV infection.
背景/目的：我们之前已证实在体内由单剪接前基因组RNA编码的一种新的剪接型乙型肝炎病毒（HBV）蛋白（HBSP）的表达。本研究旨在评估HBSP表达对HBV感染临床状态和肝脏病理的影响。
通过间接酶联免疫吸附试验检测125例慢性HBV携带者血清中HBSP抗体的存在情况。使用Knodell评分评估肝损伤的严重程度。
HBV慢性携带者中抗HBSP抗体的流行率为46%。我们强调了HBSP蛋白和抗HBSP抗体的同时表达。证实了抗HBSP抗体检测与HBV复制血清标志物之间的关联。关于HBV相关肝病，仅观察到与纤维化严重程度有关联。此外，在抗HBSP抗体阳性患者中观察到分泌型肿瘤坏死因子α（TNFα）升高，但可溶性TNFα受体75未升高。多变量分析表明，抗HBSP抗体检测与病毒复制、纤维化严重程度和TNFα分泌升高独立相关。
我们的数据提示了这样一个假说，即HBSP可能在HBV感染的自然史中起作用，并且可能参与HBV感染的发病机制和/或持续存在。
