Lemire Sharon W, Ashley David L, Calafat Antonia M
Division of Laboratory Sciences, National Center for Environmental Health/Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA.
J Anal Toxicol. 2003 Jan-Feb;27(1):1-6. doi: 10.1093/jat/27.1.1.
Nitrogen mustards are a public health concern because of their extreme vesicant properties and the possible exposure of workers during the destruction of chemical stockpiles. A sensitive, rapid, accurate, and precise analysis for the quantitation of ultratrace levels of N-ethyldiethanolamine (EDEA) and N-methyldiethanolamine (MDEA) in human urine as a means of assessing recent exposure to the nitrogen mustards bis(2-chloroethyl)ethylamine and bis(2-chloroethyl)methylamine, respectively, was developed. The method was based on solid-phase extraction, followed by analysis of the urine extract using isotope-dilution high-performance liquid chromatography-mass spectrometry with TurbolonSpray ionization and multiple-reaction monitoring. The method limits of detection were 0.41 ng/mL for EDEA and 0.96 ng/mL for MDEA in 1 mL of urine with coefficients of variation < 10% for both compounds.
氮芥因其极强的发泡特性以及在化学武器库存销毁过程中工人可能接触到而成为公共卫生问题。我们开发了一种灵敏、快速、准确且精密的分析方法，用于定量检测人尿中超痕量水平的N - 乙基二乙醇胺（EDEA）和N - 甲基二乙醇胺（MDEA），以此分别评估近期接触氮芥双（2 - 氯乙基）乙胺和双（2 - 氯乙基）甲胺的情况。该方法基于固相萃取，随后使用带有TurbolonSpray离子化和多反应监测的同位素稀释高效液相色谱 - 质谱联用仪对尿液提取物进行分析。在1 mL尿液中，EDEA的方法检测限为0.41 ng/mL，MDEA的方法检测限为0.96 ng/mL，两种化合物的变异系数均<10%。
