氧化应激对大鼠肝脏和肾脏中血清及抗氧化酶的影响及其通过饮食因素的调节作用。

Effect of oxidative stress on serum and antioxidant enzymes in liver and kidney of rats and their modulation through dietary factors.

作者信息

Ramachandran H D, Narasimhamurthy K, Raina P L

机构信息

Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore 570 013, India.

出版信息

Indian J Exp Biol. 2002 Sep;40(9):1010-5.

PMID:12587729

Abstract

Modulatory effect of a formulated diet based on cereals, pulses and spices incorporated with crude palm oil (CPO), soybean oil (SBO) or cod liver oil (CLO) at 10% dietary level on oxidative stress and antioxidant enzymes was studied in liver and kidney tissues. Activity of alkaline phosphatase (ALP) and acid phosphatase (ACP) increased significantly in serum in various experimental groups. Significant increase in hepatic antioxidant enzymes, catalase, glutathione peroxidase (GPx) was also seen in the experimental groups. SOD activity showed a mixed response. Further, kidney antioxidant enzymes did not show much change compared to those in liver. The results indicated dietary lipid as the key players in determining cellular susceptibility to oxidative stress, which could be modulated by cereals, pulses and spices in the diet.

摘要

研究了在日粮水平为10%时，基于谷物、豆类和香料并添加粗棕榈油（CPO）、大豆油（SBO）或鱼肝油（CLO）的配方日粮对肝脏和肾脏组织氧化应激及抗氧化酶的调节作用。各实验组血清中碱性磷酸酶（ALP）和酸性磷酸酶（ACP）的活性显著增加。实验组肝脏中的抗氧化酶过氧化氢酶、谷胱甘肽过氧化物酶（GPx）也显著增加。超氧化物歧化酶（SOD）活性呈现混合反应。此外，与肝脏相比，肾脏抗氧化酶变化不大。结果表明日粮中的脂质是决定细胞对氧化应激易感性的关键因素，而日粮中的谷物、豆类和香料可对其进行调节。