内皮细胞中ephrin-B2正向信号和EphB4反向信号的不同作用。
Distinct roles of ephrin-B2 forward and EphB4 reverse signaling in endothelial cells.
作者信息
Hamada Koichi, Oike Yuichi, Ito Yasuhiro, Maekawa Hiromitsu, Miyata Keishi, Shimomura Taizo, Suda Toshio
机构信息
Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto, Japan.
出版信息
Arterioscler Thromb Vasc Biol. 2003 Feb 1;23(2):190-7. doi: 10.1161/01.atv.0000055440.89758.c2.
OBJECTIVE
The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively, and bidirectional signals mediated by both proteins play an important role in vascular development. However, how such bidirectional signals are required for cell-cell adhesion or repulsion remains unclear.
METHODS AND RESULTS
Using a cell line and sorted primary endothelial cells, we show that ephrin-B2 forward signaling through the EphB4 receptor inhibits cell adhesion, whereas EphB4 reverse signaling by the transmembrane ephrin-B2 ligand does not. Cell migration is also inhibited on immobilized ephrin-B2-Fc but not on EphB4-Fc protein.
CONCLUSIONS
Ephrin-B2 forward signaling and EphB4 reverse signaling differentially affect cell adhesion and migration between arterial and venous endothelial cells.
目的
跨膜配体 Ephrin-B2 及其受体酪氨酸激酶 EphB4 分别在动脉和静脉内皮细胞上特异性表达，这两种蛋白介导的双向信号在血管发育中起重要作用。然而，这种双向信号如何参与细胞间黏附或排斥尚不清楚。
方法与结果
利用细胞系和分选的原代内皮细胞，我们发现 Ephrin-B2 通过 EphB4 受体的正向信号传导抑制细胞黏附，而跨膜 Ephrin-B2 配体介导的 EphB4 反向信号传导则无此作用。细胞迁移在固定化的 Ephrin-B2-Fc 上受到抑制，但在 EphB4-Fc 蛋白上不受影响。
结论
Ephrin-B2 正向信号传导和 EphB4 反向信号传导对动脉和静脉内皮细胞之间的细胞黏附和迁移有不同影响。
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