Navia Benjamin, Stout John, Atkins Gordon
Department of Natural Sciences, Loma Linda University, Loma Linda, California 92350, USA.
J Exp Zool A Comp Exp Biol. 2003 Mar 1;296(1):63-71. doi: 10.1002/jez.a.10242.
The L3 auditory interneuron in female Acheta domesticus, produces two different responses to the male calling song: an immediate response and a prolonged response. The prolonged response exhibited spiking activity and a correlated prolonged depolarization, both of which are clearly seen in intracellular recordings. The morphology revealed by intracellular staining was clearly the L3 neuron. The amplitude of the prolonged depolarization associated with the prolonged response increased with increases in sound intensity, resulting in increased spiking rates. Both depolarization and sound presentation increased the spiking rate and the slope of pre-potentials (thus leading to spiking threshold more quickly). Injecting hyperpolarizing current had the expected opposite effect. The effects of positive current injection and sound presentation were additive, resulting in spiking rates that were approximately double the rates in response to sound alone. Short postsynaptic potentials (PSPs), whose duration ranged from 15-60 ms, which may lead to action potentials were also observed in all recordings and summated with the prolonged depolarization, increasing the probability of spiking.
雌性家蟋蟀中的L3听觉中间神经元对雄性求偶鸣声产生两种不同反应：即时反应和延长反应。延长反应表现出峰电位活动和相关的延长去极化，这两者在细胞内记录中都清晰可见。通过细胞内染色揭示的形态明确是L3神经元。与延长反应相关的延长去极化幅度随声音强度增加而增大，导致峰电位发放率增加。去极化和声音呈现都增加了峰电位发放率和电位前斜率（从而更快达到峰电位阈值）。注入超极化电流产生预期的相反效果。注入正向电流和声音呈现的效果是相加的，导致峰电位发放率约为仅对声音反应时发放率的两倍。在所有记录中还观察到持续时间为15 - 60毫秒的短突触后电位（PSP），其可能引发动作电位，并与延长去极化相加，增加了峰电位发放的概率。
