Zieger W, Meckies J, Eichler H, Melchert F, Bugert P
Department of Gynecology and Obstetrics, Faculty of Clinical Medicine Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany.
Arch Gynecol Obstet. 2003 Feb;267(4):230-2. doi: 10.1007/s00404-002-0337-0. Epub 2002 Aug 28.
The collection of related allogenic cord blood is gaining increasing importance in families with one child affected by haematopoietic disease. Within a family, there is only a 25% chance of a full HLA match between siblings. 50% of all collected cord blood samples cannot be used because of poor quality. Because of this, the determination of HLA type is useful for planning the collection of related allogenic cord blood transplants. We studied whether HLA typing is possible during late pregnancy if amniocentesis has not been performed during the first trimester. HLA -A, -B and -DRB loci were detected in amniotic fluid, as well as in corresponding cord blood and maternal blood using PCR-SSP. For the first time, HLA typing was performed from uncultured amniocytes. Unambiguous results were obtained from all samples. Fetal HLA-genotype in amniotic fluid was confirmed by typing results from corresponding cord blood. HLA typing of uncultured amniocytes during late pregnancy is a reliable and fast method. For the first time, prenatal HLA typing by amniocentesis after week 38 of gestation is possible in less than 8 h and without fetal risk.
对于有一个孩子患造血系统疾病的家庭而言，收集相关的异基因脐血正变得越来越重要。在一个家庭中，兄弟姐妹之间完全HLA匹配的几率仅为25%。所有采集的脐血样本中有50%因质量差而无法使用。因此，HLA分型对于规划相关异基因脐血移植的采集很有用。我们研究了如果在孕早期未进行羊膜穿刺术，孕晚期是否可以进行HLA分型。使用PCR-SSP在羊水以及相应的脐血和母血中检测HLA-A、-B和-DRB基因座。首次从未培养的羊水细胞中进行HLA分型。所有样本均获得了明确的结果。羊水内胎儿的HLA基因型通过相应脐血的分型结果得到证实。孕晚期从未培养的羊水细胞进行HLA分型是一种可靠且快速的方法。首次实现了在妊娠38周后通过羊膜穿刺术在不到8小时内进行产前HLA分型，且无胎儿风险。
