[重组人骨形态发生蛋白2对人牙周膜成纤维细胞的生物学效应]
[The biological effects of recombinant human bone morphogenetic protein 2 on human periodontal ligament fibroblasts].
作者信息
Si Xiaohui, Liu Zheng
机构信息
Ninth People's Hospital, Shanghai Second Medical University.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2002 Feb;20(1):10-3.
OBJECTIVE
The purpose of this study is to study the biological effects of recombinant human bone morphogenetic protein 2 (rhBMP2) on human periodontal ligament fibroblasts (HPDLFs).
METHODS
Human periodontal ligament fibroblasts were primary cultured and detected the different doses of rhBMP2 on their proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and formation of the mineralized nodules.
RESULTS
rhBMP2 (0.25-2 mg/ml) had no remarkable effect on the proliferation of HPDLFs. The ALP activity, OC synthesis and formation of the mineralized nodules of HPDLFs were significantly stimulated by 0.5-2 mg/ml rhBMP2.
CONCLUSION
The effects of rhBMP2 on HPDLFs are dose-dependent. Not only can rhBMP2 stimulate the expression but also promote the maturation of osteoblastic phenotype of HPDLFs.
目的
本研究旨在探讨重组人骨形态发生蛋白2（rhBMP2）对人牙周膜成纤维细胞（HPDLFs）的生物学效应。
方法
原代培养人牙周膜成纤维细胞，检测不同剂量rhBMP2对其增殖、碱性磷酸酶（ALP）活性、骨钙素（OC）合成及矿化结节形成的影响。
结果
rhBMP2（0.25 - 2 mg/ml）对HPDLFs的增殖无显著影响。0.5 - 2 mg/ml rhBMP2可显著刺激HPDLFs的ALP活性、OC合成及矿化结节形成。
结论
rhBMP2对HPDLFs的作用具有剂量依赖性。rhBMP2不仅能刺激HPDLFs成骨细胞表型的表达，还能促进其成熟。
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