Song Yingliang, Xu Junwu, Ma Xuanxiang
Stomatology College, Fourth Military Medical University.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2002 Feb;20(1):14-7.
The objective of this study was to investigate the tarnish induced by Streptococcus sobrinus serotype g on Co-Cr, Ni-Cr and casting titanium alloys.
Co-Cr, Ni-Co and casting titanium alloys were respectively machined into a size of 10 mm x 10 mm x 1 mm in tablets, and totally 90 specimens with 30 of each were prepared for the test. The 30 specimens of each alloy were randomly and equally divided into three subgroups, including the blank control group, the media control group and the inoculated media group. Under the aerobic condition, the surfaces of the sucrose agar media were inoculated with Streptococcus sobrinus serotype g. The labeled sterile specimens were placed on the surface of the media for 10 weeks. The specimens were changed to a new sucrose media with bacteria each week. The specimens were removed after 10 weeks of incubation, immersed in 0.05% glutaraldehyde solution to eliminate AaY4, rinsed with distilled water. After 2 weeks, the specimens were removed and observed visually or examined by MINOLTA CR-100 color apparatus.
Compared with the blank control, the control media without bacteria did not influence the alloy specimens (P > 0.05). Streptococcus sobrinus serotype g caused tarnish on specimens. There was significant difference between the control media group and the blank control group with inoculated media group on all the specimens (P < 0.05).
Streptococcus sobrinus serotype g affects the color of Co-Cr, Ni-Cr and casting titanium alloys, but the color does not change from yellow-green range to red-yellow range.
本研究旨在调查远缘链球菌血清型g对钴铬合金、镍铬合金和铸造钛合金造成的腐蚀。
将钴铬合金、镍钴合金和铸造钛合金分别加工成尺寸为10mm×10mm×1mm的片状,共制备90个试样,每种合金30个用于测试。每种合金的30个试样随机等分为三个亚组,包括空白对照组、培养基对照组和接种培养基组。在有氧条件下,将远缘链球菌血清型g接种于蔗糖琼脂培养基表面。将标记好的无菌试样置于培养基表面10周。每周更换含有细菌的新鲜蔗糖培养基。培养10周后取出试样,浸入0.05%戊二醛溶液中以消除AaY4,用蒸馏水冲洗。2周后取出试样,进行肉眼观察或用美能达CR-100色彩仪检测。
与空白对照相比,不含细菌的对照培养基对合金试样无影响(P>0.05)。远缘链球菌血清型g导致试样出现腐蚀。所有试样的培养基对照组与空白对照组和接种培养基组之间存在显著差异(P<0.05)。
远缘链球菌血清型g会影响钴铬合金、镍铬合金和铸造钛合金的颜色,但颜色不会从黄绿范围变为红黄范围。