Gao Zhennan, Li Shengwei, Gao Jiarang
Department of Oral and Maxillofacial Surgery, College of Stomatology, West China University of Medical Sciences.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2002 Feb;20(1):55-7.
The purpose of this study is to investigate the synergistic effects of human tumor necrosis factor-alpha (hTNF-alpha) transfection and interferon-gamma (IFN-gamma) on the growth of tongue carcinoma cells.
The cultured Tca8113 tongue carcinoma cells was divided into 2 groups, one group was transferred with hTNF-alpha gene. Each of the 2 groups was then divided into 5 subgroups, and the subgroups were added IFN-gamma until the final IFN-gamma concentrations respectively were 0, 10, 100, and 1000 U/ml. After culturing for 48 hours, the survival rates of the all groups of cells were assayed by MTT enzymatic labeling technique, and the expression of hTNF-alpha in Tca8113 cells was observed with immunocytochemistry.
IFN-gamma did not affect the growth of Tca8113 cells without hTNF-alpha, however, the transfection of hTNF-alpha with the above different concentrations of IFN-gamma synergistically inhibited the growth of Tca8113 cells, the concentrations of IFN-gamma were positively correlated with the inhibition effects (r = 0.733, P < 0.01), the transferred Tca8113 cells displayed remarkable overexpression of hTNF-alpha, compared with the non-transferred.
IFN-gamma can enhance the inhibition of hTNF-alpha transfection on the tongue carcinoma cells.
本研究旨在探讨人肿瘤坏死因子-α（hTNF-α）转染与干扰素-γ（IFN-γ）对舌癌细胞生长的协同作用。
将培养的Tca8113舌癌细胞分为2组，一组转染hTNF-α基因。然后将这2组再各自分为5个亚组，分别向亚组中加入IFN-γ，使最终IFN-γ浓度分别为0、10、100和1000 U/ml。培养48小时后，采用MTT酶标技术检测各组细胞的存活率，并用免疫细胞化学法观察Tca8113细胞中hTNF-α的表达。
IFN-γ对未转染hTNF-α的Tca8113细胞生长无影响，然而，hTNF-α转染联合上述不同浓度的IFN-γ可协同抑制Tca8113细胞生长，IFN-γ浓度与抑制效果呈正相关（r = 0.733，P < 0.01），与未转染细胞相比，转染hTNF-α的Tca8113细胞呈现明显的hTNF-α过表达。
IFN-γ可增强hTNF-α转染对舌癌细胞的抑制作用。
