Blanco Anna Rita, La Terra Mulè Simona, Babini Gioia, Garbisa Spiridione, Enea Vincenzo, Rusciano Dario
Department of R&D, SIFI SpA, Via E. Patti 36, Lavinaio, Catania 95020, Italy.
Biochim Biophys Acta. 2003 Mar 17;1620(1-3):273-81. doi: 10.1016/s0304-4165(03)00007-2.
The objective of this paper is to assess the gelatinase production by some ocular pathogenic bacterial strains, and evaluate the ability of (-)epigallocatechin-3-gallate (EGCg) to inhibit this gelatinase activity and thus limit bacterial invasion. The effect of EGCg on bacterial gelatinase activity was tested by classic zymography methods, while its effect on bacterial invasion was evaluated through the ability of growing bacteria to liquefy and thus penetrate a semisolid gelatine substrate. It was found that EGCg inhibits bacterial gelatinases with an IC(50) of about 0.2 mM, and limits invasion of gelatinase-positive bacteria at concentrations above 2 mM. These results show for the first time that EGCg, as well as having direct antibacterial activity, can also inhibit bacterial gelatinases, thus limiting their invasion on gelatine. Possible use of EGCg is thus suggested as an adjuvant in antibacterial chemotherapy.
本文的目的是评估一些眼部致病细菌菌株产生明胶酶的情况，并评估（-）表没食子儿茶素-3-没食子酸酯（EGCg）抑制这种明胶酶活性从而限制细菌侵袭的能力。通过经典的酶谱法测试EGCg对细菌明胶酶活性的影响，而通过生长细菌液化并穿透半固体明胶底物的能力来评估其对细菌侵袭的影响。结果发现，EGCg以约0.2 mM的IC（50）抑制细菌明胶酶，并在浓度高于2 mM时限制明胶酶阳性细菌的侵袭。这些结果首次表明，EGCg除了具有直接抗菌活性外，还能抑制细菌明胶酶，从而限制它们在明胶上的侵袭。因此，建议将EGCg作为抗菌化疗的佐剂。
