Gavira J José A, Claxton Derek P, Wang Jenny H, Toh Diana, Meehan Edward J, DiGiammarino Enrico L
Laboratory for Structural Biology, The University of Alabama in Huntsville, Huntsville, Alabama, USA.
Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):544-6. doi: 10.1107/s0907444903000039. Epub 2003 Feb 21.
M7.1 is a class IV ubiquitin-conjugation enzyme (UBC) that belongs to the ubiquitination cascade in Caenorhabditis elegans. The clone for this UBC has been overexpressed in Escherichia coli and the 16.7 kDa protein was purified from the soluble fraction. M7.1 was crystallized by sitting-drop vapor diffusion in 10% ethanol, 1.5 M NaCl at 277.5 K. Crystals diffracted to 1.75 A and belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 44.3, b = 54.3, c = 60.2 A. The asymmetric unit contains a single monomer. A molecular-replacement model has been determined and refinement is in progress.
M7.1是一种IV类泛素结合酶（UBC），属于秀丽隐杆线虫中的泛素化级联反应。该UBC的克隆体已在大肠杆菌中过表达，并且从可溶性部分纯化出了16.7 kDa的蛋白质。M7.1通过坐滴气相扩散法在277.5 K下于10%乙醇、1.5 M氯化钠中结晶。晶体衍射至1.75 Å，属于正交晶系空间群P2(1)2(1)2(1)，晶胞参数a = 44.3、b = 54.3、c = 60.2 Å。不对称单元包含一个单体。已确定了分子置换模型，正在进行精修。
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