Crystallization and preliminary X-ray analysis of the auto-inhibited twitchin kinase.
作者信息
Hu S H, Lei J Y, Wilce M C, Valenzuela M R, Benian G M, Parker M W, Kemp B E
机构信息
St Vincent's Institute of Medical Research, Fitzroy, Victoria, Australia.
出版信息
J Mol Biol. 1994 Mar 4;236(4):1259-61. doi: 10.1016/0022-2836(94)90026-4.
PMID:8120901
Abstract
An auto-inhibited fragment of twitchin kinase (residues 5890 to 6262) has been crystallized by vapor diffusion techniques using polyethylene glycol 4000 as the precipitant at pH 7.25 to 7.5 at 4 degrees C. We have found that MgSO4 and glycerol were essential for large crystal growth. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit cell dimensions of a = 144.1 A, b = 168.3 A and c = 60.6 A. They are suitable for X-ray analysis and diffract to a resolution of at least 2.8 A.
摘要
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