Wieczorek Georg, Steinhoff Christine, Schulz Ralph, Scheller Marina, Vingron Martin, Ropers H-Hilger, Nuber Ulrike A
Max-Planck-Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany.
Cell Tissue Res. 2003 Feb;311(2):227-37. doi: 10.1007/s00441-002-0671-3. Epub 2003 Jan 31.
Bone marrow stromal cells (BMSC) have gained increased attention because of their multipotency and adult stem cell character. They have been shown to differentiate into other cell types of the mesenchymal lineage and also into non-mesenchymal cells. The exact identity of the original cells, which are isolated from bone marrow by their selective adherence to plastic, remains unknown to date. We have established and characterized mouse BMSC cultures and analyzed three independent samples by cDNA microarrays. The expression profile was compared with two previous expression studies of human BMSC and revealed a high degree of concordance between different techniques and species. To gain clues about the positional context and biology of the isolated cells within the bone marrow stroma, we searched our data for genes that encode proteins of the extracellular matrix, cell adhesion proteins, cytoskeletal proteins and cytokines/cytokine receptors. This analysis revealed a close association of BMSC with vascular cells and indicated that BMSC resemble pericytes.
骨髓基质细胞（BMSC）因其多能性和成体干细胞特性而受到越来越多的关注。它们已被证明可分化为间充质谱系的其他细胞类型，也可分化为非间充质细胞。通过选择性贴壁从骨髓中分离出的原始细胞的确切身份至今仍不清楚。我们建立并鉴定了小鼠BMSC培养物，并通过cDNA微阵列分析了三个独立样本。将该表达谱与之前两项关于人BMSC的表达研究进行比较，结果显示不同技术和物种之间具有高度一致性。为了获取有关分离细胞在骨髓基质中的位置背景和生物学特性的线索，我们在数据中搜索了编码细胞外基质蛋白、细胞粘附蛋白、细胞骨架蛋白以及细胞因子/细胞因子受体的基因。该分析揭示了BMSC与血管细胞的密切关联，并表明BMSC类似于周细胞。
