Yang Xiao-Fu, Huang He-Feng
The Affiliated Obstetrics and Gynecology Hospital, College of Medical Sciences, Zhejiang University, Hangzhou 310006, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2002 Jun;31(3):155-158. doi: 10.3785/j.issn.1008-9292.2002.03.005.
To investigate the impact of ovarian stimulation on leptin levels and their effect on in vitro fertilization-embryo transfer (IVF-ET). METHODS: In 39 women who underwent IVF-ET, serum and follicular fluid leptin and E(2) levels were measured by enzyme-linked immunosorbent assay and radioimmunoassay. Blood samples were collected just prior to FSH and HCG injection and follicles puncture. Follicular fluid was collected at the time of oocyte retrieval. RESULTS: The serum leptin levels before HCG administration (26.1+/-2.3)&mgr;g/L were significantly higher than before FSH administration (9.3+/-1.0)&mgr;g/L and oocyte retrieval (15.8+/-2.3)&mgr;g/L(P<0.001). On the day of oocyte retrieval, there was no significant difference between the serum leptin(15.8+/-2.3)&mgr;g/L.and the follicular fluid leptin (18.5+/-2.2)&mgr;g/L. Serum and follicular leptin levels in successful pregnancies were significantly lower(P<0.05) than in women with failed conception. CONCLUSION: It could indicate that high leptin levels may interfere with the developing of dominant follicles.
探讨卵巢刺激对瘦素水平的影响及其对体外受精-胚胎移植（IVF-ET）的作用。方法：对39例行IVF-ET的女性，采用酶联免疫吸附测定法和放射免疫测定法检测血清和卵泡液中的瘦素及E(2)水平。在注射促卵泡激素（FSH）和人绒毛膜促性腺激素（HCG）前以及卵泡穿刺时采集血样。在取卵时收集卵泡液。结果：注射HCG前血清瘦素水平（26.1±2.3）μg/L显著高于注射FSH前（9.3±1.0）μg/L及取卵时（15.8±2.3）μg/L（P<0.001）。取卵当天，血清瘦素水平（15.8±2.3）μg/L与卵泡液瘦素水平（18.5±2.2）μg/L之间无显著差异。成功妊娠者的血清和卵泡瘦素水平显著低于未受孕女性（P<0.05）。结论：这可能表明高瘦素水平可能会干扰优势卵泡的发育。
