Ito T
Department of Biochemistry, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
Curr Top Microbiol Immunol. 2003;274:1-22. doi: 10.1007/978-3-642-55747-7_1.
Packaging of the eukaryotic genome into chromatin functions not only to constrain the genome within the boundaries of the cell nucleus but also to permit dynamic and broad-ranging changes related to many important biological phenomena. Therefore, chromatin assembly is a process that affects DNA replication, repair, and gene expression. Chromatin structure is linked to transcriptional regulation, and recent studies show how chromatin is altered so as to facilitate transcription. In addition, modification of chromatin structure is an important regulatory mechanism. Here I review the mechanism of chromatin assembly in vitro and the changes of chromatin structure related to transcriptional activation.
真核生物基因组包装成染色质，其功能不仅是将基因组限制在细胞核范围内，还允许与许多重要生物学现象相关的动态和广泛变化。因此，染色质组装是一个影响DNA复制、修复和基因表达的过程。染色质结构与转录调控相关，最近的研究表明染色质是如何改变以促进转录的。此外，染色质结构的修饰是一种重要的调控机制。在此，我综述体外染色质组装机制以及与转录激活相关的染色质结构变化。
