Mercy Inderjit Singh, Meeley Robert B, Nichols Scott E, Olsen Odd-Arne
PO Box 5051, Agricultural University of Norway, 1432 Aas, Norway.
J Exp Bot. 2003 Mar;54(384):1117-9. doi: 10.1093/jxb/erg118.
A cDNA clone from a 4 DAP dissected maize embryo sac encoding a novel Zea mays single-repeat Myb protein is reported here. This full-length cDNA contains an ORF of 948 bp. The gene ZmMybst1 contains two introns (1166 and 706 bp) and is a single copy gene. The ZmMybst1 protein shares high sequence identity with the potato Mybst1 protein (58%). Northern blot, RT-PCR and electronic northern analysis shows that ZmMybst1 is expressed in endosperm between 4 and 30 DAP, coinciding with the period of aleurone cell differentiation and development.
本文报道了一个从授粉后4天解剖的玉米胚囊中获得的cDNA克隆，其编码一种新的玉米单重复Myb蛋白。这个全长cDNA包含一个948 bp的开放阅读框。ZmMybst1基因含有两个内含子（分别为1166 bp和706 bp），是一个单拷贝基因。ZmMybst1蛋白与马铃薯Mybst1蛋白具有较高的序列同一性（58%）。Northern杂交、RT-PCR和电子Northern分析表明，ZmMybst1在授粉后4至30天的胚乳中表达，这与糊粉层细胞分化和发育的时期一致。
