Shimizu Kazuhiro, Naito Shinji, Urata Yoshishige, Takamiyagi Atsushi, Bae Sang Jae, Ogawa Fumihide, Kondo Takahito, Katayama Ichiro
Department of Dermatology, Nagasaki University School of Medicine, Japan.
J Dermatol. 2003 Jan;30(1):17-25.
Gaseous carbon monoxide (CO) has received attention as a neurotransmitter and as a material involved in persistent dilatation of vessels. CO is released by heme oxygenase (HO) during the process from heme to bilirubin or biliverdin. Many reports have revealed that exogenous nitric oxide (NO) can induce HO-1 in vitro, which is the induced isoform of HO. In the present study, we attempted an ex vivo system as an explant culture. A quantitative analysis was performed in combination with a reverse transcription-competitive polymerase chain reaction method, which proved to be very accurate, as well as a qualitative analysis with an immunohistochemistry. With this system we confirmed the induction of HO-1 mRNA and protein by exogenous NO in normal human skin. Our results concluded that this ex vivo system was very useful, because skin samples could be handled easily under conditions close to the in vivo situation.
气态一氧化碳（CO）作为一种神经递质以及参与血管持续性扩张的物质受到了关注。在从血红素到胆红素或胆绿素的过程中，血红素加氧酶（HO）会释放出CO。许多报告显示，外源性一氧化氮（NO）在体外可诱导HO-1，HO-1是HO的诱导型同工酶。在本研究中，我们尝试了一种作为外植体培养的离体系统。结合一种经证明非常准确的逆转录竞争聚合酶链反应方法进行了定量分析，以及用免疫组织化学进行定性分析。通过该系统，我们证实了外源性NO可在正常人皮肤中诱导HO-1 mRNA和蛋白。我们的结果表明，这种离体系统非常有用，因为皮肤样本可以在接近体内情况的条件下轻松处理。
